ABSTRACT
β-Cyclodextrin-N-vinyl-2-pyrrolidone (CDM-NVP) copolymer was prepared by free radical chain reaction of N-vinyl-2-pyrrolidone (NVP) and β-CD maleate (CDM) with γ-ray as initiator.The synthesis conditions of CDM-NVP polymer were as follows: mass ratio of CDM to NVP was 1∶0.7 (CDM 3.6 g and NVP 2.52 g), irradiation dosage was 4 kGy, and DMF aqueous solution (50%, V/V) was set at 20 mL.Under the synthesis conditions, the yield of CDM-NVP was 84% and the weight-average molecular weight was 20 kD.The natamycin (NM) and carbendazim (MBC) could form stable inclusion complexes with CDM-NVP copolymer, and the solubility and fungicidal activity of the complexes were investigated.The stability constant of NM·CDM-NVP and MBC·CDM-NVP complexes at 303 K were 12, 988.54 L/mol and 865.94 L/mol, respectively.The complexes were characterized using phase solubility diagrams, nuclear magnetic resonance (NMR) spectra and ultraviolet (UV) spectra.The analysis of the biological activities of these two complexes indicated that they possessed enhancing fungicidal activities compared to NM and MBC alone.
ABSTRACT
ObjectiveTo investigate the genetic polymorphisms of 21 short tandem repeat(STR)loci (D3S1358, D13S317, D7S820, D16S539, Penta E, D2S441, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391 and FGA). Methods A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer.ResultsA total of 248 alleles were observed, the al-lelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium exceptPentaE(P=0.023). The combined pow-er of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10-25, respectively. ConclusionThe 21 STR loci show high polymorphisms in the Han population, which can provide valu-able data and a theoretical basis for forensic individual identification and paternity testing.